How to extract the isolated chlorogenic acid

The process flow has been produced from Eucommia leaf: 40% ethanol extraction (80 ° C, 2 h, 1: 16 binder, extraction 2 times) → Preparation of dipper (vacuum concentration, 80 ° C drying) → Add water to 40 → lead salt precipitation (1 140 g of Eucommia, lead) → analyze chlorogenic acid (20% H2SO4) → Ethyl acetate (about 20 times) → concentrated to precipitate → heavy The crystallization was purified, and the average yield was 0.53%, purity 98.35%. The Eucommia leaf is also found in the study.